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C-myc epitope tag antibody

WebSpecificity / Sensitivity. Myc-Tag Antibody detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in … WebAnti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the …

Purified anti-c-Myc Antibody anti-c-Myc - 9E10

WebEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag ... WebJan 28, 2024 · We tested by Western blotting six antibodies that recognize the c-Myc epitope tag, including monoclonal antibodies 9E10, 4A6, 9B11, and 71D10 and … damart thermolactyl vest https://mastgloves.com

Overview of Epitope Tagging Thermo Fisher Scientific - US

WebBy Stewart Newlove, PhD. Epitope tags are short peptide sequences, generally composed of 10-15 amino acids, designed to create a molecular handle for a protein. An epitope tag can be placed anywhere within a protein but typically are placed on either the C-terminus or N-terminus in order to minimise tertiary structure disruptions that may alter ... WebImmunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc. WebAnti-Myc Tag Antibody, clone 4A6 Anti-Myc Tag, clone 4A6, is a mouse monoclonal antibody that is validated for use in ChIP, IC, IF, IP, and WB for the detection of Myc Tag and does not show the very high context sensitivity of 9E10. This antibody has been cited in over 480 publications. - Find MSDS or SDS, a COA, data sheets and more information. bird island basin corpus christi

Overview of Epitope Tagging Thermo Fisher Scientific - US

Category:Direct-Blot HRP anti-c-Myc Antibody anti-c-Myc - 9E10

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C-myc epitope tag antibody

The Myc tag monoclonal antibody 9E10 displays highly

WebEpitope tags such as V5, c-myc, and HA are biological structures, usually proteins, that are genetically engineered onto either the N or C terminus of a recombinant protein. Tags function as universal epitopes, easily … WebMagnetic beads were coupled to an anti-c-myc antibody and exposed to a mixture of H1N1 and the biotinylated c-myc-tagged 238D2 (AviTag-238D2, lane 1), or the biotinylated c-myc-tagged 3SM5 (AviTag-3SM5, lane 2) or to H1N1 alone (lane 3). In lanes 4, 5, and 6 are shown the inputs. The beads eluate, and the input samples were analyzed by Western ...

C-myc epitope tag antibody

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WebMonoclonal antibodies are produced by injecting an antigen into a host animal, in order to initiate a humoral immune response, and by subsequently extracting the antibody-producin WebStudy c-Myc binding partners and target genes with our highly validated antibodies and high purity inhibitors. MAX antibodies and inhibitors: Mouse monoclonal [73C5a] to MAX - ChIP Grade ab53570. Rabbit polyclonal to Max (phospho S11) ab191493. Cell permeable c-Myc-Max dimerization inhibitor (10058-F4) ab145065.

WebCatalog-Num Product Name Quantity Price Supplier Category; Catalog-Num Product Name Quantity Price Supplier; NBN Monoclonal Antibody [3E4] (100 ug) 569-Eur: EpigenTek: … WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-Myc tag antibody [9E10] (ab206486) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – confirmed specificity ...

WebSep 27, 2024 · Epitope tags are widely used in various applications, but often lack versatility. ... Ramsay, G. & Bishop, J. M. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product ... WebPurified anti-c-Myc Antibody - The c-myc protein is a 62 kD nuclear factor that is ubiquitously expressed in the nucleus. ... (250 µg total protein) prepared from HeLa cells mock-transfected (-) or transfected (+) with multi-tag plasmid containing c-Myc epitope sequence were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ ...

WebProduct Details. Endogenous Human c-Myc; fusion protein with c-Myc tag (EQKLISEEDL, around 1.2kD) in all species. This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives. The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

WebThe anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are … bir discount codeWebPurified anti-c-Myc Antibody - The c-myc protein is a 62 kD nuclear factor that is ubiquitously expressed in the nucleus. ... (250 µg total protein) prepared from HeLa cells … bird island flathead lakeWebJan 28, 2024 · All three rabbit antibodies showed a preference for the Myc epitope tag in a C-terminal position (in the contexts of vectors pCMV-Myc C and pcDNA3.1/Myc). In particular, antibody 9106 (Abcam) displayed a strong bias for the C-terminal tag position, with the pCMV-Myc C context protein about eight times better detected than the pCMV … damart womens fleece trousers