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Atarf5

WebAuxin is a key regulator that virtually controls almost every aspect of plant growth and development throughout its life cycle. As the major components of auxin signaling, auxin response factors (ARFs) play crucial roles in various processes of plant growth and development. In this study, a total of 35 PtrARF genes were identified, and their … WebApr 7, 2011 · For example, arf mutations caused the change in gynoecium patterning (AtARF3) [17–19], impaired hypocotyls response to blue light, growth and auxin sensitivity (AtARF7) [20–24], formation of vascular strands and embryo axis formation (AtARF5) , suppression of hookless phenotype and hypocotyl bending (AtARF2) [26–28], hypocotyl …

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WebA. thaliana ARF5 (AtARF5) is the first plant ARF gene isolated by map-based cloning, and it plays an important role in the formation of embryo pattern and vascular tissue (Hardtke & Berleth, 1998). Mutations in AtARF1 and AtARF2 affect the growth patterns of pistils, as well as leaf senescence, floral organ abscission ( Ellis et al., 2005 ). WebSep 22, 2024 · In the root, NsARF5 shows significantly increased expression, while AtARF5 is similar to NsARF5 in the phylogenetic tree, and it has been confirmed in Arabidopsis that AtARF5 is very important in the formation of the radicle . It is suspected that NsARF5 is crucial in the control of the root growth and development in N. sibirica. incoloy 825 welding https://mastgloves.com

Key auxin response factor (ARF) genes constraining wheat

Webmechanistic action of the A. thaliana ARF5 and ARF1 DBDs (AtARF5 and AtARF1 respectively; Boer, et al. 2014), and these same ARF domains served as a selection scaffold for the GmARF8a DBD. Boer et al. expressed the DNA binding domains of AtARF1 and AtARF5 proteins, incorporating residues 1-354 and 1-390, respectively. WebOct 16, 2024 · The canonical AuxRE ER7 had significantly higher binding to AtARF5-DBD versus the above AuxREs while ER7-GG had the highest binding to AtARF5-DBD. Therefore, we concluded that the SYBR Gold-based assay can be used to quantify relative affinities of different protein-DNA interactions. WebOct 5, 2024 · At the molecular level, MP/AtARF5 had been identified as a central regulator in leaf vascularization (Krogan and Berleth, 2012; Krogan et al., 2012) and root growth (Krogan et al., 2016). In M. sieboldii , vascular tissue differentiation was preliminarily observed after stratification for 30 days. incoloy 903 specs

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Category:Genome-wide characterization of the auxin response factor (ARF ... - PeerJ

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Atarf5

CmRCD1 repressed CmBBX8 activity. PC: positive control …

WebNov 14, 2024 · Overall, these results provide evidence that AtARF2–AtARF4 and AtARF5 play significant roles in regulating both female and male gametophyte development in Arabidopsis. Introduction The life cycle of plants includes alternating generations of a diploid sporophyte and a highly reduced haploid gametophyte. WebPC: positive control (AtARF5); NC: negative control (empty vector). a Structure of the vector. b Fluorescence images; from blue to red, the fluorescence value increases gradually. c Relative LUC ...

Atarf5

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WebSuperimposition of onemonomerof AtARF5-DBD (class A; yellow, PDB code 4LDU) on AtARF1-DBD (blue). (f) Superimposition of a model of one monomer of AtARF10-DBD (class C; orange with its DD2 in red) on AtARF1-DBD(blue).(g–i)Structuralmodelsof the AtARF5-DBD dimer docked with IR7 (g), DR5 (h) and ER13 (i). The structures were WebOct 21, 2024 · Results indicate that auxin response factors AtARF2-AtARF4 and AtARF5 play significant roles in regulating both female and male gametophyte development. regulation establishes the threshold at which leaves respond to a temporal signal, rather than being a component of this signal.

WebIn the composite model, the AtARF5 dimer (green) binds two everted TGTCTC motifs (AuxRE) separated by 7-bp (ER7 oligonucleotide) via its B3-type subdomains ( Korasick et al., 2015b). Dimerization ... Web98 AtARF5 clade (ZmARF4 and ZmARF29) and the AtARF19 clade (ZmARF27). We found that 99 Class A clade ARFs across species largely shared promoter preferences, and additionally found 100 that AtARF19 was the only ARF tested to be able to activate transcription on promoters with a 101 single AuxRE.

WebAug 25, 2024 · Previous reports showed that AtARF5 is an activator with super-strong activities and is usually used as a positive control for the activity analysis of transcriptional activators [46, 47]. 35S::GAL4DB-AtARF5 protoplasts showed high … WebDec 17, 2024 · AtARF5 influences embryo, root, and shoot development (Krogan et al., 2012; Crawford et al., 2015). AtARF9 acts in suspensor cells to mediate hypophysis specification ( Rademacher et al., 2012 ), and AtARF10/16/17 play vital roles in negatively regulating seed germination and post-germination activities ( Liu et al., 2007 ).

WebMay 19, 2024 · AtARF5 participates in the maintenance of apical meristem development by directly regulating AtARR7 and AtARR15, and is involved in the development of leaf vascular bundle tissue by regulating AtATHB8 [22, 23]. In tomato, ARFs play important roles in fruit development and maturation.

WebNational Center for Biotechnology Information incoloy 909 hardnessWebApr 17, 2024 · Auxin signaling and modules of interaction. (a) Structure of the AtAux/IAA17 PB1 (red)–AtARF5 PB1 (yellow) complex (PDB codes 6L5K and 4CHK). (b) AtTPL N-terminus (orange) in complex with the AtAux/IAA27 EAR motif (red sticks; PDB code 5NQV). (c) AtTIR1 (green)–auxin (green spheres)–AtAux/IAA7 degron (red sticks) complex (PDB … incoloy 825 sheetWebJul 1, 2024 · A 7.5 μg aliquot of either p35S::GAL4DB-AtARF5 (positive control), p35S::GAL4DB (negative control) or p35S::GAL4DB-CmMYB2 (test construct) was introduced into the protoplasts. incoloy 825 chemistry